Journal: bioRxiv

Article Title: Single-cell transcriptomics identifies regulation of invasive behavior in Drosophila follicle cells with polarity loss

doi: 10.1101/2022.06.09.495554

Figure Lengend Snippet: A. UMAP Plot of subdivided cluster 7 cells, superimposed with RNA velocity vectors. B. Correlation heatmap representing the Connection Specificity Index (CSI) matrix of active regulon modules. C. Heatmap of the unscaled activity scores of regulons in the subclusters of cluster 7. D. Heatmap of the differentially expressed markers in each subclusters of cluster 7 cells, scaled within +1.5 (red) and -1.5 (blue) log 2 fold change. E. Gene enrichment plot for cnc (red), Keap1 (green) and their overlap (bottom). F. Phase portraits showing dynamic behavior of genes in cluster 7 cells (colored according to subcluster identity as shown in ). Solid line represents the learned splicing dynamics while the dotted line represents the inferred steady state of gene expression. Keap1 , GstD1 and GstD2 exhibits increased transcription in cluster 7. G. Reporter expression of GstD-lacZ is detected by β-Gal expression (red) within the multilayered cells of lgl RNAi follicle cells (green; clonal boundaries are marked by the dotted white lines). Nuclei is marked by DAPI (white). Scale bars: 20 µm.

Article Snippet: The following antibodies or dyes are mentioned in this paper: DSHB: mouse anti-Arm (N27A1, 1:40 dilution), mouse anti-Cut (2B10, 1:30), rat anti-Shg (DCAD2, 1:20), mouse anti-Hnt (1G9, 1:15), rat anti-Mmp1 (1:1:1 mixture of 3B8, 3A6 and 5H7, 1:40), mouse anti-Sn (sn7C, 1:25). mouse anti-Drpr (5D14, 1:50) Promega: mouse anti-β-Gal (PAZ3783, 1:500).

Techniques: Activity Assay, Expressing

Journal: bioRxiv

Article Title: Single-cell transcriptomics identifies regulation of invasive behavior in Drosophila follicle cells with polarity loss

doi: 10.1101/2022.06.09.495554

Figure Lengend Snippet: A. Confocal Images showing GstD-lacZ expression, marked by β-Gal staining (red), in border cells of w 1118 egg chambers. Nucleus is marked by DAPI (white). Scale bars, 20 µm.

Article Snippet: The following antibodies or dyes are mentioned in this paper: DSHB: mouse anti-Arm (N27A1, 1:40 dilution), mouse anti-Cut (2B10, 1:30), rat anti-Shg (DCAD2, 1:20), mouse anti-Hnt (1G9, 1:15), rat anti-Mmp1 (1:1:1 mixture of 3B8, 3A6 and 5H7, 1:40), mouse anti-Sn (sn7C, 1:25). mouse anti-Drpr (5D14, 1:50) Promega: mouse anti-β-Gal (PAZ3783, 1:500).

Techniques: Expressing, Staining

Journal: Aging Cell

Article Title: Local Growth Hormone Facilitates Aging of the Colon Epithelial Microenvironment

doi: 10.1111/acel.70187

Figure Lengend Snippet: The npGH is expressed in senescent cells, and autocrine and paracrine GH promote β‐catenin nuclear translocation. (A‐B) Representative confocal images of npGH expression in senescent cells in (A) normal colon and (B) hyperplastic colon polyp. SA‐β‐gal, green; GH, red; colocalization, yellow. Scale bar = 100 μm. (C) Representative confocal image of hNCC expressing lentiGH. Arrow indicates GFP‐positive cell expressing GH (green) and active β‐catenin (red). Arrowhead indicates the cell cultured in proximity of a GH‐expressing cell also expressing intranuclear active β‐catenin. Scale bar = 20 μm. (D‐F) Representative cross‐sections of confocal Z‐stacks of hNCC showing localization of active β‐catenin (red) and nucleus (blue). (D) Active β‐catenin is outside the nucleus in GFP‐positive lentiV hNCC, (E) in the nucleus of GFP‐positive lentiGH hNCC, and (F) in the nucleus of GFP‐negative hNCC cocultured with lentiGH hNCC.

Article Snippet: Tissues were permeabilized in 1% Triton X100 for 30 min, followed by blocking in 10% goat serum for 1 h. Tissues were stained overnight at 4°C with anti β‐gal mouse monoclonal antibodies (dilution 1:50) and anti‐hGH rabbit antibodies (dilution 1:100), both from LSBio (cat#LS‐ B10989 and cat#LS‐B4199, respectively) followed by goat anti‐mouse Alexa 488 and goat anti‐rabbit Alexa 568 secondary antibodies, both from Invitrogen (cat#A32723 and #A11031, respectively).

Techniques: Translocation Assay, Expressing, Cell Culture